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Plant Tissue Culture Contamination Control Additive

Plant Tissue Culture Contamination Control Additive

https://www.cytolyva.com/web/image/product.template/1099215/image_1920?unique=699b802
REF: CTLV-0001-02
Size: 100 mL

£ 113.19 113.19 GBP £ 113.19

113.19 €

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Intended UseStorage and StabilityTips for Best ResultsGeneral ProtocolRecommended UsageCompatible Culture MediaApplicationsKey FeaturesOverview

Overview

Plant Tissue Culture Contamination Control Additive is a non-sterile laboratory media supplement designed to support the establishment and maintenance of in-vitro plant tissue culture systems.

During plant micropropagation workflows, contamination originating from explants, handling, or environmental exposure can disrupt culture development. When incorporated into culture media, this additive helps maintain stable culture conditions and supports successful plant tissue culture establishment.

The reagent is suitable for use in solid or liquid plant culture media and is compatible with most standard plant tissue culture formulations used in research laboratories and micropropagation facilities.

This product is supplied non-sterile and is intended exclusively for laboratory plant tissue culture applications.

Key Features

  • Supports stable conditions in plant tissue culture systems
  • Compatible with most standard plant culture media formulations
  • Effective at low working concentrations
  • Suitable for both solid and liquid culture media
  • Compatible with standard media preparation workflows
  • Useful when working with challenging explant material

Applications

Plant Micropropagation

Supports stable culture conditions during shoot multiplication and rooting stages in plant tissue culture systems.


Explant Establishment

Facilitates the establishment of cultures from plant material collected from greenhouse or field environments.


Callus Culture

Suitable for callus induction experiments where consistent culture conditions are important for successful tissue development.


Plant Regeneration Studies

Applicable in plant regeneration protocols involving organogenesis or somatic embryogenesis.


Plant Biotechnology Research

Useful in plant transformation workflows and other experimental procedures involving plant tissue culture.

Compatible Culture Media

The additive can be used with most commonly employed plant tissue culture formulations, including:


  • Murashige & Skoog (MS) medium
  • Gamborg B5 medium
  • Woody Plant Medium (WPM)
  • Chu N6 medium
  • Custom plant tissue culture media​

Recommended Usage

The additive is typically used at low concentrations during plant tissue culture media preparation.

Culture Stage

Suggested Concentration

Explant establishment

0.1–0.2 % (v/v)

Routine micropropagation

0.05–0.1 % (v/v)

Sensitive plant material

optimize experimentally​

Optimal concentrations may vary depending on plant species, explant source, and laboratory conditions.

General Protocol

1. Prepare Culture Medium

Prepare the plant tissue culture medium according to the desired formulation.


2. Add the Additive

Add the Plant Tissue Culture Contamination Control Additive to the medium at the desired final concentration.


3. Mix Thoroughly

Mix the medium to ensure even distribution of the additive.


4. Sterilization

Sterilize the medium according to standard laboratory procedures appropriate for the selected medium formulation.


5. Dispense Medium

Pour the prepared medium into sterile culture vessels.


6. Introduce Plant Material

Transfer plant explants to the prepared medium using standard sterile tissue culture techniques.

Tips for Best Results

  • Perform small-scale tests when working with new plant species.
  • Combine proper explant surface sterilization with appropriate culture practices.
  • Adjust additive concentration depending on plant species and culture conditions.

Storage and Stability

  •  Store according to the conditions indicated on the product label
  • Avoid repeated contamination of the stock solution during routine laboratory use
  • Use appropriate aseptic handling practices due to non-sterile formulation

Intended Use

  • For research and laboratory plant tissue culture use only.
  • Not intended for agricultural field use, environmental applications, or crop protection purposes.
  Murashige & Skoog Basal Salts With Macronutrients and Micronutrients   Product OverviewApplicationsProduct DescriptionPreparation Guidance Storage and Handling ​ Specifications ​ Suggested Use ​ Why Choose CytoLyva Murashige & Skoog Basal Salts?

  Murashige & Skoog Basal Salts With Macronutrients and Micronutrients  

 

Powdered basal salt formulation for plant tissue culture, micropropagation and plant research applications.

CytoLyva's Murashige & Skoog Basal Salts With Macronutrients and Micronutrients is a powdered plant tissue culture basal salt formulation developed for laboratories involved in plant cell culture, tissue culture, micropropagation, regeneration and plant physiology research.

The formulation contains essential macronutrients and micronutrients used in Murashige & Skoog (MS)-based plant culture media. Each bottle contains 2,215 Kg of powder, sufficient to prepare 500 litres of basal salt solution when reconstituted according to the appropriate laboratory protocol.

Product Overview

Murashige & Skoog (MS) medium is one of the most widely used basal media in plant tissue culture. This formulation provides the macronutrients and micronutrients of the MS basal salt system for use in the in vitro cultivation of a wide variety of plant species.

The powdered format enables researchers to prepare fresh basal medium while maintaining flexibility to modify the final formulation according to the plant species, tissue type and experimental requirements.

Depending on the intended application, the prepared basal medium may require supplementation with:

  • Vitamins
  • Sucrose or another suitable carbon source
  • Agar or other gelling agents
  • Plant growth regulators, such as auxins and cytokinins
  • Antibiotics, when required
  • Activated charcoal
  • Other laboratory supplements

Applications

CytoLyva Murashige & Skoog Basal Salts With Macronutrients and Micronutrients may be used for the preparation of media for:

  • Plant tissue culture
  • Plant cell culture
  • Micropropagation
  • In vitro seed germination
  • Callus induction and maintenance
  • Shoot induction and multiplication
  • Root induction
  • Plant regeneration
  • Culture of explants, meristems and plant organs
  • Plant physiology studies
  • Agricultural and horticultural research
  • Botanical research
  • Preparation of customized plant culture media

Product Description

Murashige & Skoog basal salts provide essential mineral nutrients used for the controlled in vitro cultivation of plant tissues, cells and organs.

This formulation contains MS macronutrients and micronutrients in a convenient powdered format, allowing laboratories to prepare a basal salt solution and supplement it according to their specific plant culture protocols.

The formulation includes:

  • Macronutrients, which provide mineral elements required in relatively large quantities
  • Micronutrients, which provide essential trace elements required in smaller quantities

The performance of the final prepared medium depends on several experimental variables, including:

  • Plant species
  • Explant type
  • Culture conditions
  • Medium pH
  • Vitamin supplementation
  • Plant growth regulators
  • Carbon source
  • Additional supplements

Researchers may adapt the basal formulation according to their specific experimental protocols.

CytoLyva's powdered formulation provides a convenient solution for laboratories routinely preparing MS-based plant culture media.

Preparation Guidance

Dissolve the complete contents of one 2,215 Kg bottle in purified or distilled water and bring to a final volume of 500 litres.

The exact preparation procedure may vary according to the laboratory protocol. Researchers may need to:

  • Dissolve the powder in approximately 80–90% of the required final water volume.
  • Add vitamins if required by the selected formulation.
  • Add sucrose or another suitable carbon source if required.
  • Supplement with plant growth regulators or other additives as appropriate.
  • Adjust the pH according to the selected protocol.
  • Add a suitable gelling agent when preparing solid medium.
  • Bring the solution to the final volume of 500 L.
  • Sterilize the prepared medium using an appropriate validated laboratory procedure.

Heat-sensitive supplements should normally be sterilized separately by filtration and added aseptically after sterilization and cooling of the basal medium, where appropriate.

The optimal supplementation, pH and sterilization conditions should be validated for the intended plant species and application.

Storage and Handling


Store the unopened product under the conditions indicated on the product label or accompanying technical documentation.

Keep the container tightly sealed and protect the powder from:

  • Moisture
  • Excessive heat
  • Direct sunlight

Where applicable, allow the container to reach room temperature before opening to help minimize condensation.

Handle the product using appropriate laboratory procedures, personal protective equipment and good laboratory practice.

​ Specifications

Product Name: Murashige & Skoog Basal Salts With Macronutrients and Micronutrients

Reference: CTLV-0135-05

Form: Powder

Composition: Murashige & Skoog Macronutrients and Micronutrients

Net Weight: 2,215 Kg

Preparation Volume: 500 L

Application: Plant tissue culture and plant research

Intended Use: Research Use Only

​ Suggested Use

Murashige & Skoog Basal Salts With Macronutrients and Micronutrients provide the mineral nutrient foundation for numerous plant tissue culture applications.

The basal salt formulation may be supplemented with vitamins, a carbon source, plant growth regulators and other components depending on the intended application, including:

  • Callus induction
  • Shoot multiplication
  • Rooting
  • Plant regeneration
  • Organogenesis
  • Somatic embryogenesis

The optimal final medium composition depends on:

  • Plant species
  • Cultivar
  • Explant type
  • Developmental stage
  • Vitamin supplementation
  • Growth regulator combination
  • Carbon source
  • Culture temperature
  • Light conditions
  • Liquid or solid culture system
  • Experimental objectives

Researchers should optimize the final medium formulation for their specific experimental system.

​ Why Choose CytoLyva Murashige & Skoog Basal Salts?

CytoLyva provides plant tissue culture reagents designed for convenient and consistent laboratory medium preparation.

This Murashige & Skoog Basal Salts With Macronutrients and Micronutrients formulation provides the mineral components of MS basal salts in a single pre-weighed 2,215 Kg bottle, sufficient to prepare 500 litres of basal salt solution.

Its powdered format allows laboratories to prepare fresh media while maintaining flexibility to add vitamins, carbon sources, plant growth regulators and other supplements according to specific experimental requirements.

The formulation is suitable for use in a broad range of plant tissue culture, micropropagation, regeneration, plant physiology, agricultural, horticultural and botanical research applications.