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Plant Tissue Culture Contamination Control Additive

Plant Tissue Culture Contamination Control Additive

https://www.cytolyva.com/web/image/product.template/1099235/image_1920?unique=085af0d
REF: CTLV-0001-05
Size: 1 L

£ 1,013.02 1013.02 GBP £ 1,013.02

1,013.02 €

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OverviewKey FeaturesApplicationsCompatible Culture MediaRecommended UsageGeneral ProtocolTips for Best ResultsStorage and StabilityIntended Use

Overview

Plant Tissue Culture Contamination Control Additive is a laboratory media supplement designed to support the establishment and maintenance of in-vitro plant tissue culture systems.


During plant micropropagation workflows, contamination originating from explants, handling, or environmental exposure can disrupt culture development. When incorporated into culture media, this additive helps maintain stable culture conditions and supports successful plant tissue culture establishment.


The reagent is suitable for use in solid or liquid plant culture media and is compatible with most standard plant tissue culture formulations used in research laboratories and micropropagation facilities.


This product is intended exclusively for laboratory plant tissue culture applications.

Key Features

  • Supports stable conditions in plant tissue culture systems
  • Compatible with most standard plant culture media formulations
  • Effective at low working concentrations
  • Suitable for both solid and liquid culture media
  • Compatible with standard media preparation workflows
  • Useful when working with challenging explant material

Applications

Plant Micropropagation

Supports stable culture conditions during shoot multiplication and rooting stages in plant tissue culture systems.


Explant Establishment

Facilitates the establishment of cultures from plant material collected from greenhouse or field environments.


Callus Culture

Suitable for callus induction experiments where consistent culture conditions are important for successful tissue development.


Plant Regeneration Studies

Applicable in plant regeneration protocols involving organogenesis or somatic embryogenesis.


Plant Biotechnology Research

Useful in plant transformation workflows and other experimental procedures involving plant tissue culture.

Compatible Culture Media

The additive can be used with most commonly employed plant tissue culture formulations, including:


  • Murashige & Skoog (MS) medium
  • Gamborg B5 medium
  • Woody Plant Medium (WPM)
  • Chu N6 medium
  • Custom plant tissue culture media​

Recommended Usage

The additive is typically used at low concentrations during plant tissue culture media preparation.

Culture Stage

Suggested Concentration

Explant establishment

0.1–0.2 % (v/v)

Routine micropropagation

0.05–0.1 % (v/v)

Sensitive plant material

optimize experimentally​

Optimal concentrations may vary depending on plant species, explant source, and laboratory conditions.

General Protocol

1. Prepare Culture Medium

Prepare the plant tissue culture medium according to the desired formulation.


2. Add the Additive

Add the Plant Tissue Culture Contamination Control Additive to the medium at the desired final concentration.


3. Mix Thoroughly

Mix the medium to ensure even distribution of the additive.


4. Sterilization

Sterilize the medium according to standard laboratory procedures appropriate for the selected medium formulation.


5. Dispense Medium

Pour the prepared medium into sterile culture vessels.


6. Introduce Plant Material

Transfer plant explants to the prepared medium using standard sterile tissue culture techniques.

Tips for Best Results

  • Perform small-scale tests when working with new plant species.
  • Combine proper explant surface sterilization with appropriate culture practices.
  • Adjust additive concentration depending on plant species and culture conditions.

Storage and Stability

  •  Store according to the conditions indicated on the product label.
  • Avoid repeated contamination of the stock solution during routine laboratory use.

Intended Use

  • For research and laboratory plant tissue culture use only.
  • Not intended for agricultural field use, environmental applications, or crop protection purposes.
Murashige & Skoog Basal Salts with Macronutrients, Micronutrients, Vitamins and Glycine   Product OverviewApplicationsProduct DescriptionSpecificationsPreparation Guidance Storage and Handling ​ Suggested Use ​ Why Choose CytoLyva Murashige & Skoog Basal Salts?

Murashige & Skoog Basal Salts with Macronutrients, Micronutrients, Vitamins and Glycine  

Complete powdered basal medium for plant tissue culture, micropropagation and plant research applications.

CytoLyva’s Murashige & Skoog Basal Salts with Macronutrients, Micronutrients, Vitamins and Glycine is a complete powdered plant tissue culture medium designed for laboratories working with plant cell culture, tissue culture, micropropagation, regeneration and plant physiology research.

The formulation contains the essential mineral salts, vitamins and glycine commonly required to support plant tissue growth under controlled laboratory conditions. Each pouch contains 221.30 g of powder, sufficient to prepare 50 litres of basal medium when reconstituted according to the appropriate laboratory protocol.

Product Overview

Murashige & Skoog medium, commonly referred to as MS medium, is one of the most widely used basal media in plant tissue culture. It provides a balanced combination of macronutrients, micronutrients, vitamins and glycine to support the in vitro growth and development of a broad range of plant species.

This powdered formulation allows researchers to prepare fresh culture medium and adapt the final composition according to the requirements of the plant species, tissue type and experimental protocol.

Depending on the application, researchers may supplement the prepared medium with sucrose, agar or another gelling agent, plant growth regulators, antibiotics or other experimental additives.

Applications

CytoLyva Murashige & Skoog Basal Salts may be used in:

  • Plant tissue culture
  • Plant cell culture
  • Micropropagation
  • In vitro seed germination
  • Callus induction and maintenance
  • Shoot induction and multiplication
  • Root induction
  • Plant regeneration workflows
  • Culture of explants, meristems and plant organs
  • Plant physiology and developmental studies
  • Agricultural, horticultural and botanical research
  • Preparation of customized plant culture media

Product Description

Murashige & Skoog basal medium provides essential inorganic nutrients and vitamins required for the controlled in vitro cultivation of plant cells, tissues and organs.

The macronutrient components supply elements required in relatively large quantities for plant growth, while the micronutrients provide essential trace elements. The included vitamins and glycine support cellular metabolism and tissue development under laboratory culture conditions.

The final performance of the prepared medium may depend on the plant species, explant type, pH, culture conditions and additional supplements used. Researchers can modify the basal medium by adding plant growth regulators such as auxins or cytokinins, as well as sucrose, gelling agents or other compounds required for a specific experimental workflow.

CytoLyva’s powdered format offers a practical solution for laboratories that require consistent preparation of MS-based plant culture medium.

Specifications

  • Product Name: Murashige & Skoog Basal Salts with Macronutrients, Micronutrients, Vitamins and Glycine
  • Reference: CTLV-0139-04
  • Form: Powder
  • Composition: Macronutrients, micronutrients, vitamins and glycine
  • Pack Size: 1 Pouch
  • Net Weight:221.30 g
  • Preparation Volume: 50 L
  • Application: Plant tissue culture and plant research
  • Intended Use: Research use only

Preparation Guidance

Dissolve the complete contents of one 221.30 g pouch in purified or distilled water to prepare a final volume of 50 litres.

The exact preparation procedure may vary according to the laboratory protocol. Researchers may need to:

  1. Dissolve the powder in approximately 80–90% of the required final water volume.
  2. Add sucrose, plant growth regulators or other supplements as required.
  3. Adjust the pH according to the selected plant tissue culture protocol.
  4. Add a suitable gelling agent when preparing solid medium.
  5. Bring the solution to the final volume of 50 L.
  6. Sterilize or filter the prepared medium according to the requirements of the added components and experimental procedure.

The optimal supplementation, pH and sterilization conditions should be validated for the specific plant species and tissue culture application.

Storage and Handling

Store the unopened product according to the conditions indicated on the product label or accompanying technical documentation.

Keep the bottle tightly closed and protect the powder from moisture, excessive heat and direct light. Allow the container to reach room temperature before opening when necessary to help reduce the risk of condensation.

Handle the product using standard laboratory procedures, appropriate personal protective equipment and good laboratory practice.

​ Suggested Use

Murashige & Skoog Basal Salts are commonly used as the nutritional foundation for plant tissue culture media. The basal formulation can be adapted by adding appropriate plant growth regulators and supplements according to the required response, such as callus induction, shoot multiplication, rooting or plant regeneration.

The optimal medium composition may vary depending on:

  • Plant species and cultivar
  • Explant or tissue type
  • Developmental stage
  • Plant growth regulator combination
  • Culture temperature and lighting
  • Liquid or solid culture format
  • Experimental objectives

Researchers should validate the final formulation and culture conditions for their specific experimental system.

​ Why Choose CytoLyva Murashige & Skoog Basal Salts?

CytoLyva provides plant research reagents designed for consistency, convenient preparation and reliable integration into laboratory workflows.

This MS basal salts formulation combines macronutrients, micronutrients, vitamins and glycine in one pre-weighed pouch. With 221.30 g sufficient for preparing 50 litres, it offers a practical solution for laboratories performing plant tissue culture, micropropagation, regeneration and plant physiology research.