Indole-3-Butyric Acid (IBA)

Product Overview

Murashige & Skoog (MS) medium is one of the most widely used basal media in plant tissue culture. This formulation provides the macronutrients and micronutrients of the MS basal salt system for use in the in vitro cultivation of a wide variety of plant species.

The powdered format enables researchers to prepare fresh basal medium while maintaining flexibility to modify the final formulation according to the plant species, tissue type and experimental requirements.

Depending on the intended application, the prepared basal medium may require supplementation with:

• Vitamins
• Sucrose or another suitable carbon source
• Agar or other gelling agents
• Plant growth regulators, such as auxins and cytokinins
• Antibiotics, when required
• Activated charcoal
• Other laboratory supplements

Applications

CytoLyva Murashige & Skoog Basal Salts With Macronutrients and Micronutrients may be used for the preparation of media for:

• Plant tissue culture
• Plant cell culture
• Micropropagation
• In vitro seed germination
• Callus induction and maintenance
• Shoot induction and multiplication
• Root induction
• Plant regeneration
• Culture of explants, meristems and plant organs
• Plant physiology studies
• Agricultural and horticultural research
• Botanical research
• Preparation of customized plant culture media

Product Description

Murashige & Skoog basal salts provide essential mineral nutrients used for the controlled in vitro cultivation of plant tissues, cells and organs.

This formulation contains MS macronutrients and micronutrients in a convenient powdered format, allowing laboratories to prepare a basal salt solution and supplement it according to their specific plant culture protocols.

The formulation includes:

• Macronutrients, which provide mineral elements required in relatively large quantities
• Micronutrients, which provide essential trace elements required in smaller quantities

The performance of the final prepared medium depends on several experimental variables, including:

• Plant species
• Explant type
• Culture conditions
• Medium pH
• Vitamin supplementation
• Plant growth regulators
• Carbon source
• Additional supplements

Researchers may adapt the basal formulation according to their specific experimental protocols.

CytoLyva's powdered formulation provides a convenient solution for laboratories routinely preparing MS-based plant culture media.

Preparation Guidance

Dissolve the complete contents of one 2,215 Kg bottle in purified or distilled water and bring to a final volume of 500 litres.

The exact preparation procedure may vary according to the laboratory protocol. Researchers may need to:

• Dissolve the powder in approximately 80–90% of the required final water volume.
• Add vitamins if required by the selected formulation.
• Add sucrose or another suitable carbon source if required.
• Supplement with plant growth regulators or other additives as appropriate.
• Adjust the pH according to the selected protocol.
• Add a suitable gelling agent when preparing solid medium.
• Bring the solution to the final volume of 500 L.
• Sterilize the prepared medium using an appropriate validated laboratory procedure.

Heat-sensitive supplements should normally be sterilized separately by filtration and added aseptically after sterilization and cooling of the basal medium, where appropriate.

The optimal supplementation, pH and sterilization conditions should be validated for the intended plant species and application.

Storage and Handling

Store the unopened product under the conditions indicated on the product label or accompanying technical documentation.

Keep the container tightly sealed and protect the powder from:

• Moisture
• Excessive heat
• Direct sunlight

Where applicable, allow the container to reach room temperature before opening to help minimize condensation.

Handle the product using appropriate laboratory procedures, personal protective equipment and good laboratory practice.

​ Specifications

Product Name: Murashige & Skoog Basal Salts With Macronutrients and Micronutrients

Reference: CTLV-0135-05

Form: Powder

Composition: Murashige & Skoog Macronutrients and Micronutrients

Net Weight: 2,215 Kg

Preparation Volume: 500 L

Application: Plant tissue culture and plant research

Intended Use: Research Use Only

​ Suggested Use

Murashige & Skoog Basal Salts With Macronutrients and Micronutrients provide the mineral nutrient foundation for numerous plant tissue culture applications.

The basal salt formulation may be supplemented with vitamins, a carbon source, plant growth regulators and other components depending on the intended application, including:

• Callus induction
• Shoot multiplication
• Rooting
• Plant regeneration
• Organogenesis
• Somatic embryogenesis

The optimal final medium composition depends on:

• Plant species
• Cultivar
• Explant type
• Developmental stage
• Vitamin supplementation
• Growth regulator combination
• Carbon source
• Culture temperature
• Light conditions
• Liquid or solid culture system
• Experimental objectives

Researchers should optimize the final medium formulation for their specific experimental system.

​ Why Choose CytoLyva Murashige & Skoog Basal Salts?

CytoLyva provides plant tissue culture reagents designed for convenient and consistent laboratory medium preparation.

This Murashige & Skoog Basal Salts With Macronutrients and Micronutrients formulation provides the mineral components of MS basal salts in a single pre-weighed 2,215 Kg bottle, sufficient to prepare 500 litres of basal salt solution.

Its powdered format allows laboratories to prepare fresh media while maintaining flexibility to add vitamins, carbon sources, plant growth regulators and other supplements according to specific experimental requirements.

The formulation is suitable for use in a broad range of plant tissue culture, micropropagation, regeneration, plant physiology, agricultural, horticultural and botanical research applications.