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Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Vitamins Without Glycine

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REF: CTLV-0136-01
Size: 5 L

£ 34.64 34.64 GBP £ 34.64

34.64 €

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Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Gamborg's Vitamins

Complete powdered basal medium for plant tissue culture, micropropagation and plant research applications.

CytoLyva's Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Gamborg's Vitamins is a complete powdered plant tissue culture medium developed for laboratories involved in plant cell culture, tissue culture, micropropagation, regeneration and plant physiology research.

The formulation contains the essential macronutrients, micronutrients and Gamborg's vitamins required for the in vitro cultivation of a wide variety of plant species. Each bottle contains 221.50 g of powder, sufficient to prepare 50 litres of basal medium when reconstituted according to the appropriate laboratory protocol.

Product Overview


Murashige & Skoog (MS) medium is one of the most widely used basal media in plant tissue culture. This formulation combines a balanced mixture of macronutrients, micronutrients and Gamborg's vitamins to support the in vitro growth and development of numerous plant species.

The powdered format enables researchers to prepare fresh culture medium while allowing flexibility to modify the final composition according to the plant species, tissue type and experimental requirements.

Depending on the intended application, the prepared medium may be supplemented with:

  • Sucrose
  • Agar or other gelling agents
  • Plant growth regulators (auxins and cytokinins)
  • Antibiotics
  • Activated charcoal
  • Other laboratory supplements

Applications


CytoLyva Murashige & Skoog Basal Salts with Gamborg's Vitamins may be used in:

  • Plant tissue culture
  • Plant cell culture
  • Micropropagation
  • In vitro seed germination
  • Callus induction and maintenance
  • Shoot induction and multiplication
  • Root induction
  • Plant regeneration
  • Culture of explants, meristems and plant organs
  • Plant physiology studies
  • Agricultural and horticultural research
  • Botanical research
  • Preparation of customized plant culture media

Product Description


Murashige & Skoog basal medium provides essential mineral nutrients required for the controlled in vitro cultivation of plant tissues, cells and organs. In this formulation, the MS basal salts are combined with Gamborg's vitamins to provide a convenient complete powdered medium for laboratory preparation.

The formulation includes:

  • Macronutrients required in relatively large quantities for plant growth
  • Micronutrients supplying essential trace elements
  • Gamborg's vitamins to support cellular metabolism and in vitro plant culture

The final performance of the prepared medium depends on several experimental variables, including:

  • Plant species
  • Explant type
  • Culture conditions
  • Medium pH
  • Added plant growth regulators
  • Carbon source
  • Additional supplements

Researchers can adapt the basal formulation according to their own experimental protocols.

CytoLyva's powdered formulation provides a convenient and reproducible solution for laboratories routinely preparing MS-based plant culture media.

Preparation Guidance


Dissolve the complete contents of one 221.50 g bottle in purified or distilled water to prepare a final volume of 50 litres.

The preparation procedure may vary according to laboratory protocols. Researchers may need to:

  • Dissolve the powder in approximately 80–90% of the required final water volume.
  • Add sucrose if required.
  • Supplement with plant growth regulators or other additives.
  • Adjust the pH according to the selected protocol.
  • Add a suitable gelling agent when preparing solid media.
  • Bring the solution to the final volume of 50 L.
  • Sterilize or filter the prepared medium according to the requirements of the added components and experimental procedure.

The optimal supplementation, pH and sterilization conditions should always be validated for the intended plant species and application.

Storage and Handling


Store the unopened product under the conditions indicated on the product label or accompanying technical documentation.

Keep the pouch tightly sealed and protect the powder from:

  • Moisture
  • Excessive heat
  • Direct sunlight

Allow the container to reach room temperature before opening when necessary to minimize condensation.

Handle the product using appropriate laboratory procedures, personal protective equipment and good laboratory practice.

​ Specifications


Product Name: Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Gamborg's Vitamins

Reference: CTLV-0137-04

Form: Powder

Composition: Macronutrients, Micronutrients and Gamborg's Vitamins

Pack Size: 1 Bottle

Net Weight: 221.50 g

Preparation Volume: 50 L

Application: Plant tissue culture and plant research

Intended Use: Research Use Only

​ Suggested Use


Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Gamborg's Vitamins serve as the nutritional foundation for numerous plant tissue culture applications.

The basal formulation may be customized through the addition of plant growth regulators and other supplements depending on the desired application, including:

  • Callus induction
  • Shoot multiplication
  • Rooting
  • Plant regeneration
  • Organogenesis
  • Somatic embryogenesis

The optimal medium composition depends on:

  • Plant species
  • Cultivar
  • Explant type
  • Developmental stage
  • Growth regulator combination
  • Culture temperature
  • Light conditions
  • Liquid or solid culture system
  • Experimental objectives

Researchers should optimize the final formulation for their specific experimental system.

​ Why Choose CytoLyva Murashige & Skoog Basal Salts?


CytoLyva provides high-quality plant tissue culture reagents designed for reliable laboratory performance and consistent medium preparation.

This Murashige & Skoog Basal Salts With Macronutrients, Micronutrients and Gamborg's Vitamins formulation combines essential mineral nutrients and Gamborg's vitamins in a single pre-weighed 221.50 g bottle, sufficient to prepare 50 litres of culture medium.

Its convenient powdered format allows laboratories to prepare fresh media while maintaining flexibility to optimize formulations for plant tissue culture, micropropagation, regeneration, plant physiology and other plant research applications.