Product Overview

Murashige & Skoog (MS) medium is one of the most widely used basal media in plant tissue culture. This modified formulation provides the macronutrients and micronutrients of the MS basal salt system, without ammonium nitrate or potassium nitrate, for preparing media suitable for various in vitro plant culture applications.

The powdered format allows researchers to prepare fresh basal media while maintaining the flexibility to adjust the final formulation according to plant species, tissue type, and experimental requirements.

The absence of ammonium nitrate and potassium nitrate allows researchers to investigate different nitrogen and potassium supplementation strategies and adjust mineral nutrient concentrations according to their experimental objectives.

Depending on the intended application, the prepared basal medium may require the addition of:

• Vitamins

• Sucrose or another suitable carbon source

• Agar or other gelling agents

• Plant growth regulators, such as auxins and cytokinins

• Alternative nitrogen and potassium sources, if necessary

• Antibiotics, when required by the protocol

• Activated charcoal

• Other laboratory supplements

Applications

CytoLyva's Murashige & Skoog Basal Salts with Macronutrients and Micronutrients, Without Ammonium Nitrate or Potassium Nitrate, can be used to prepare media for the following applications:

• Plant tissue culture

• Plant cell culture

• Micropropagation

• In vitro seed germination

• Callus induction and maintenance

• Shoot induction and multiplication

• Root induction

• Plant regeneration

• Explant, meristem, and plant organ culture

• Plant physiology studies

• Nitrogen metabolism and mineral nutrition studies

• Agricultural and horticultural research

• Botanical research

• Preparation of customized plant culture media

Product Description

Murashige & Skoog basal salts provide the mineral nutrients used for the controlled in vitro culture of plant tissues, cells, and organs.

This modified formulation contains MS macronutrients and micronutrients, without ammonium nitrate or potassium nitrate, in a convenient powdered form, allowing laboratories to prepare a basal salt solution and supplement it according to their specific plant culture protocols.

The formulation includes:

• Macronutrients: Components supplying mineral nutrients required in relatively large quantities, excluding ammonium nitrate and potassium nitrate.

• Micronutrients: Components supplying trace elements required in smaller quantities.

The exclusion of ammonium nitrate and potassium nitrate allows researchers to adjust nitrogen supplementation and investigate the effects of different nutritional conditions on plant growth and development.

The performance of the final prepared medium depends on several experimental variables, including:

• Plant species

• Explant type

• Culture conditions

• Medium pH

• Vitamin supplementation

• Plant growth regulators

• Carbon source

• Nitrogen and potassium availability

• Additional supplements

Researchers can adapt the basal formulation according to their specific experimental protocols.

CytoLyva's powdered formulation provides a practical solution for laboratories that regularly prepare modified MS-based plant culture media.

Preparation Guidance

Use the 221.50 g bottle, following the manufacturer's preparation instructions, to obtain the declared final volume of 50 liters.

The nominal quantity corresponds to 4.43 g of powder per liter of prepared medium.

The exact preparation procedure may vary depending on the laboratory protocol. Researchers may need to:

1. Dissolve the specified quantity of powder in approximately 80–90% of the required final volume of water.

2. Mix until the components are dissolved, following the preparation instructions.

3. Add vitamins if required by the selected formulation.

4. Add sucrose or another suitable carbon source, if necessary.

5. Supplement with alternative nitrogen or potassium sources when required by the experimental protocol.

6. Add plant growth regulators or other additives as needed.

7. Adjust the pH according to the selected protocol.

8. Add a suitable gelling agent when preparing a solid medium.

9. Bring the solution to the final volume of 50 L.

10. Sterilize the prepared medium using an appropriate, validated laboratory procedure.

Heat-sensitive supplements should generally be sterilized separately by filtration and added aseptically after sterilization and cooling of the basal medium, when appropriate.

Optimal supplementation, pH, and sterilization conditions should be adapted to the intended plant species and application.

Note: Because ammonium nitrate and potassium nitrate are excluded, additional nutrient supplementation may be necessary depending on the intended application. Powder quantities and preparation volumes must follow the manufacturer's formulation and preparation instructions.

Storage and Handling

Store the unopened product under the conditions specified on the label or in the accompanying technical documentation.

Keep the container tightly closed and protect the powder from:

• Moisture

• Excessive heat

• Direct sunlight

When appropriate, allow the container to reach room temperature before opening to minimize condensation.

Handle the product according to appropriate laboratory procedures, using the necessary personal protective equipment and following good laboratory practices.

​ Specifications

​ Suggested Use

Murashige & Skoog Basal Salts with Macronutrients and Micronutrients, Without Ammonium Nitrate or Potassium Nitrate, provide a modified mineral base for various plant tissue culture applications.

The basal salt formulation can be supplemented with vitamins, a carbon source, alternative nitrogen sources, plant growth regulators, and other components depending on the intended application, including:

• Callus induction

• Shoot multiplication

• Rooting

• Plant regeneration

• Organogenesis

• Somatic embryogenesis

The optimal composition of the final medium depends on the following factors:

• Plant species

• Cultivar

• Explant type

• Developmental stage

• Vitamin supplementation

• Plant growth regulator combinations

• Carbon source

• Nitrogen and potassium availability

• Culture temperature

• Lighting conditions

• Liquid or solid culture system

• Experimental objectives

Researchers should optimize the final medium formulation according to their specific experimental system.

​ Why Choose CytoLyva Murashige & Skoog Basal Salts with Macronutrients and Micronutrients, Without Ammonium Nitrate or Potassium Nitrate?

CytoLyva offers plant tissue culture reagents designed to facilitate laboratory medium preparation.

This formulation of Murashige & Skoog Basal Salts with Macronutrients and Micronutrients, Without Ammonium Nitrate or Potassium Nitrate, provides a modified mineral base in a single 221.50 g bottle, with a declared preparation volume of 50 liters.

Its powdered format allows laboratories to prepare fresh media while maintaining the flexibility to add vitamins, carbon sources, alternative nitrogen sources, plant growth regulators, and other supplements according to specific experimental requirements.

The absence of ammonium nitrate and potassium nitrate makes this formulation particularly suitable for research requiring modified nitrogen supplementation and customized nutritional conditions.

The 50 L format is especially suitable for laboratories that regularly prepare plant culture media and require sufficient powder for larger preparation volumes.

This formulation can be used in a wide range of research applications involving plant tissue culture, micropropagation, plant regeneration, plant physiology, mineral nutrition, agriculture, horticulture, and botany.